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An investigation of the factors leading to false results of haemoglobin testing with luminol


Abstract
Luminol, being very sensitive, is often used in crime scenes to detect traces of blood. However, the crime scene could have been cleaned up before the forensic department arrives. Our project is focused on finding out whether cleaning products would affect the results of the haemoglobin testing with luminol. However as blood could not be obtained, iron (II) sulfate was used as a substitute instead. There were two sets of tests, one was carried out by soaking 3 cloths that have iron (II) sulfate on it, into solutions of diluted bleach, detergent and soap. The other was soaking 3 pieces of cloths into the same solutions of diluted bleach, detergent and soap, without iron (II) sulfate. After the cloths has been taken out and dried, it is tested with luminol. The results are then compared and analysed. Based on the results, we can conclude that bleach and detergent might give a false result as they both glowed with and without “blood”. In the future, this research could lead to more accurate testing in crime scene investigations that uses luminol.

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